Telomere length (TL) has been proposed as a biomarker of biological age that may assist to estimate individual trajectories of aging. Individuals age at remarkably different rates so that the health status and functional impairment can vary widely at the same chronological age.
Age-related differences in TERF-1 and TERF-2 expression were inconsistent.Ĭonclusions: The present study questions the utility of RTL in PBMCs as a biomarker for the individual assessment of aging. mRNA expression of TERF-1 and TERF-2 was tissue-specific with the highest levels in liver. Age-related differences in TERT expression were found in PBMCs, skeletal muscle, and visceral fat. The expression of TERT significantly differed between the tested organs with highest levels in liver and kidney. A significant difference of RTL between young and aged animals was only observed in aorta (0.98 ± 0.15 vs. Results: Mean RTL in PBMCs and solid tissues of young rats ranged from 0.64 ± 0.26 in large intestine to 1.07 ± 0.22 in skeletal muscle. The mRNA expression of telomerase (TERT) and shelterin proteins TERF-1 and TERF-2 was also measured. Methods: Relative TL (RTL) was measured in PBMCS and multiple solid tissues from 24 young (4 months) and 24 aged (14 months) Sprague-Dawley (SD) rats. Solid evidence that supports this concept is lacking. Telomere length (TL) in peripheral blood mononuclear cells (PBMCs) has been proposed as surrogate marker for TL in the entire organism. Background: Telomeres are protective nucleoprotein structures at the end of chromosomes that shorten with age.
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